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Description
Methods similar to those now being used for human DNA fingerprinting are similarly applicable to the conclusive identification of wine grape cultivars. This approach offers advantages over conventional ampelographic identification in that environmental influences on morphology as well as differences in human judgement are eliminated. Highly purified DNA was extracted from 43 accessions of34 wine grape cultivars, including most major California varieties, and subjected to restriction fragment length polymorphism analysis. Several DNA probes that were isolated from a grape DNA library produced in our laboratory were used to produce highly informative complex DNA banding patterns, similar in appearance and concept to a bar code. The combined results from eight probes produced a unique pattern for each cultivar analyzed. These results predict that the probability that two cul ti vars of Vitis vinifera would share the same patterns between 1 and 34 000, and 1 in 700 billion, depending on the cultivars, suggesting that this method has the discriminatory power to uniquely identify any cultivar. Preliminary comparisons of accessions of Zinfandel and Primitivo di Gioia revealed identical DNA patterns, confirming the hypothesis that these varieties are identical. Additional comparisons of other varieties will be presented.
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