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Sound / DNA fingerprinting of some California wine grape cultivars

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Title
DNA fingerprinting of some California wine grape cultivars
Creator
Bowers, John E
Date Created and/or Issued
1992
Publication Information
American Society for Enology and Viticulture
Contributing Institution
Sonoma County Library
Collection
Wine and Viticulture Conference and Workshop Presentations
Rights Information
Copyrighted. Rights are owned by the American Society for Enology and Viticulture. The Sonoma County Library makes no assertions as to ownership of any original copyrights to digitized work and can claim only physical ownership of the work(s) described in these records. However, these materials are intended for Personal or Research use only. Any other kind of use, including, but not limited to commercial or scholarly publication in any medium or format, public exhibition, or use online or in a web site, may be subject to additional restrictions including but not limited to the copyrights held by parties other than the Library. USERS ARE SOLELY RESPONSIBLE for determining the existence of such rights and for obtaining any permissions and/or paying associated fees necessary for the proposed use. Preferred credit line is: Courtesy, the Sonoma County Library. Please see <a href='https://sonomalibrary.org/locations/sonoma-county-history-and-genealogy-library/order-photo' target='_blank' rel='noopener noreferrer'>additional reproduction and reuse information</a>
Description
Methods similar to those now being used for human DNA fingerprinting are similarly applicable to the conclusive identification of wine grape cultivars. This approach offers advantages over conventional ampelographic identification in that environmental influences on morphology as well as differences in human judgement are eliminated. Highly purified DNA was extracted from 43 accessions of34 wine grape cultivars, including most major California varieties, and subjected to restriction fragment length polymorphism analysis. Several DNA probes that were isolated from a grape DNA library produced in our laboratory were used to produce highly informative complex DNA banding patterns, similar in appearance and concept to a bar code. The combined results from eight probes produced a unique pattern for each cultivar analyzed. These results predict that the probability that two cul ti vars of Vitis vinifera would share the same patterns between 1 and 34 000, and 1 in 700 billion, depending on the cultivars, suggesting that this method has the discriminatory power to uniquely identify any cultivar. Preliminary comparisons of accessions of Zinfandel and Primitivo di Gioia revealed identical DNA patterns, confirming the hypothesis that these varieties are identical. Additional comparisons of other varieties will be presented.
Type
sound
Format
conference proceedings
lectures
Identifier
e2c83efe-d333-4e62-95c2-2e2adad9bfff
https://digital.sonomalibrary.org/documents/detail/504045
https://images.quartexcollections.com/sonomalibrary/thumbnails/preview/e2c83efe-d333-4e62-95c2-2e2adad9bfff
cstr_aud_000037
Language
English
Subject
Viticulture
Grapes--Genetics
DNA fingerprinting
Relation
Wine and Viticulture Conference and Workshop Presentations

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