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Moving Image / CIL:15792, Strongylocentrotus purpuratus

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Title
CIL:15792, Strongylocentrotus purpuratus
Creator
Bement, William M
Miller, Ann L
Sider, Jenny R
Verbrugghe, Koen J.C
Von Dassow, George
Contributor
Bement, William M.
Miller, Ann L.
Sider, Jenny R.
Verbrugghe, Koen J.C.
Von Dassow, George
Date Created and/or Issued
2021
Contributing Institution
UC San Diego, Research Data Curation Program
Collection
Cell Image Library
Rights Information
Under copyright
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Rights Holder and Contact
UC Regents
Description
Untreated 16-cell purple urchin embryo with EMTB-3G (microtubules) in shown cyan and mC-H2B (chromatin) in yellow.). The video shows normal correlation between aster development, furrowing, and the extent of separation of sister chromatids. Embryos were imaged with a laser scanning confocal microscope (Radiance 2000; Bio-Rad Laboratories) mounted on a compound microscope (E800; Nikon) using a 40× 1.3 NA Plan-Fluor oil lens. Room temperature was maintained at 14–17°C. The video corresponds to the first segment of video 5 and Fig. S3A in J Cell Biol. 2009 Dec 14;187(6):831-45. Note this video is the control video for an embryo treated with trichostatin A (TSA), an inhibitor of HDAC6 (a tubulin deacetylase) that selectively disrupts dynamic microtubules in mammalian cells that is also part of this image group.
Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
Von Dassow, George; Verbrugghe, Koen J.C.; Miller, Ann L.; Sider, Jenny R.; Bement, William M. (2021). CIL:15792, Strongylocentrotus purpuratus. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J04B301Q
Type
moving image
Identifier
ark:/20775/bb03820697
Language
No linguistic content
Subject
Cytokinesis after mitosis
Embryo
Cleavage furrow
Ensconsin
Microtubule
Histone
Aster
Strongylocentrotus purpuratus
Cell Image Library Group ID: 6184

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