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Description
Untreated 16-cell purple urchin embryo with EMTB-3G (microtubules) in shown cyan and mC-H2B (chromatin) in yellow.). The video shows normal correlation between aster development, furrowing, and the extent of separation of sister chromatids. Embryos were imaged with a laser scanning confocal microscope (Radiance 2000; Bio-Rad Laboratories) mounted on a compound microscope (E800; Nikon) using a 40× 1.3 NA Plan-Fluor oil lens. Room temperature was maintained at 14–17°C. The video corresponds to the first segment of video 5 and Fig. S3A in J Cell Biol. 2009 Dec 14;187(6):831-45. Note this video is the control video for an embryo treated with trichostatin A (TSA), an inhibitor of HDAC6 (a tubulin deacetylase) that selectively disrupts dynamic microtubules in mammalian cells that is also part of this image group. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Von Dassow, George; Verbrugghe, Koen J.C.; Miller, Ann L.; Sider, Jenny R.; Bement, William M. (2021). CIL:15792, Strongylocentrotus purpuratus. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J04B301Q
Type
moving image
Identifier
ark:/20775/bb03820697
Language
No linguistic content
Subject
Cytokinesis after mitosis Embryo Cleavage furrow Ensconsin Microtubule Histone Aster Strongylocentrotus purpuratus Cell Image Library Group ID: 6184
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