Under copyright Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work. Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Rights Holder and Contact
UC Regents
Description
Transmission electron micrograph of Drosophila nephrocyte garland cell. Wild-type garland cells display extensive tubulo-vesicular and vacuolar profiles of endosomal compartments, including electron-lucent alpha vacuoles akin to early endosomes in the cell cortex, and electron-dense beta vacuoles that represent late endosomes more centrally. The largest vacuoles in wild type are 2 microns in diameter and all vacuoles contain a single aggregate of electron-dense granular material that appears to be attached to the limiting membrane. Samples for TEM were fixed for 1 h at 4°C in 2% paraformaldehyde, 2% glutaraldehyde, and 1% tannic acid in 0.1 M cacodylic acid buffer, pH 7.2. Then, the samples were postfixed in 1% OsO4 in 0.1 M cacodylic acid buffer, pH 7.2, for 1 h at room temperature, stained en bloc with 1% uranyl acetate, dehydrated in a grade series of ethanol and propylene oxide, and embedded in epon resin (Electron Microscopy Sciences). Blocks were sectioned in an ultramicrotome (RMC Products) at ∼70-nm thickness with a Delaware Diamond knife and post-stained for 1 h in Reynolds lead citrate and uranyl acetate. Electron micrographs were taken on a transmission electron microscope (H-7500; Hitachi) using an AMT camera system. Mag: 5000x. Image corresponds to Figure 2B, left WT panel in Kim et al. J Cell Biol. 188: 717-734. 2010. A high magnification view of this region is in CIL# 13471. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Kim, Sungsu; Wairkar, Yogesh P.; Daniels, Richard W.; DiAntonio, Aaron (2021). CIL:13470, Drosophila melanogaster, garland cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0HH6HRM J Cell Biol. 188: 717-734. 2010 https://www.ncbi.nlm.nih.gov/pubmed/?term=20194640
Type
image
Language
No linguistic content; Not applicable
Subject
Endocytosis Late endosome Nucleus Early endosome Garland cell Drosophila melanogaster Cell Image Library Group ID: 6022
If you're wondering about permissions and what you can do with this item, a good starting point is the "rights information" on this page. See our terms of use for more tips.
Share your story
Has Calisphere helped you advance your research, complete a project, or find something meaningful? We'd love to hear about it; please send us a message.
