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Description
RCC-4 cell expressing wild-type Von Hippel-Lindau protein (pVHL30) stained with anti-alpha-tubulin (green) and anti-GTP-tubulin (red) antibodies. GTP-tubulin localizes to the microtubule (MT) tip and remnants of GTP-tubulin are found along the entire MT lattice. When pVHL30 is expressed in RCC-4 cells, a higher frequency of GTP caps and GTP remnants is observed relative to RCC-4 control (VHL negative). RCC-4 cells were permeabilized in PEM buffer with glycerol and 0.1% Triton-X100, then incubated with primary antibody hMB11 followed by incubation with secondary antibody. Cells were then fixed in cold methanol for 5 min and stained with the polyclonal anti-tubulin antibody followed by secondary antibody. Stacks of images were acquired with an IX70 Delta Vision Spectris microscope using a 60X NA1.4 DIC oil Plan-Apochromat objective. This stack corresponds to J Cell Biol. 2010. 190: 991-1003--Fig 5F (right 2 panels) and the control (left 2 panels in Fig 5F) is CIL# 26572. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Thoma, Claudio R.; Matov, Alexandre; Gutbrodt, Katrin L.; Hoerner, Christian R.; Smole, Zlatko; Krek, Wilhelm; Danuser, Gaudenz (2021). CIL:26572, Homo sapiens, epithelial cell, renal cell carcinoma. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0J101X8
Type
image
Identifier
ark:/20775/bb0996453j
Language
No linguistic content
Subject
Protein polymerization Protein binding RCC4 Structural molecule activity GTPase activity Microtubule Renal cell carcinoma Epithelial cell Cytoskeleton Protein complex Homo sapiens Cell Image Library Group ID: 8073
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