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Image / CIL:23042, Rattus, fibroblast

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Title
CIL:23042, Rattus, fibroblast
Creator
Borisy, Gary G
Svitkina, Tatyana M
Verkhovsky, Alexander B
Contributor
Borisy, Gary G.
Svitkina, Tatyana M.
Verkhovsky, Alexander B.
Date Created and/or Issued
2021
Contributing Institution
UC San Diego, Research Data Curation Program
Collection
Cell Image Library
Rights Information
Under copyright
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Rights Holder and Contact
UC Regents
Description
Association of plectin with myosin II. Platinum replica of nocodazole and treated REF-52 cell with actin depleted by gelsolin and plectin labeled with immunogold (10 nm). Individual plectin molecules in lamella cross-link myosin filaments and their clusters. Some plectin molecules bind to each other. Electron microscopy of cytoskeletons was performed as described (Svitkina et al., 1995). Briefly, cells on coverslips were lysed as for light microscopy, treated, with recombinant gelsolin NHz-terminal domain, fixed with glutaraldehyde, tannic acid and uranyl acetate, critical point dried, and coated with platinum and carbon. Image corresponds to Fig 10e from J Cell Biol. 1996 Nov;135(4):991-1007.
Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
Svitkina, Tatyana M.; Verkhovsky, Alexander B.; Borisy, Gary G. (2021). CIL:23042, Rattus, fibroblast. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0NS0SQ6
Type
image
Identifier
ark:/20775/bb1064682m
Language
No linguistic content
Subject
Gelsolin treatment
REF-52
Nocodazole treatment
Plectin
Fibroblast
Myosin II complex
Rattus
Cell Image Library Group ID: 8924

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