Bennetzen, Martin v Bénit, Paule Cabrera, Sandra Criollo, Alfredo Eisenberg, Tobias Galluzzi, Lorenzo Horio, Yoshiyuki Kepp, Oliver Maiuri, Maria Chiara Malik, Shoaib Ahmad Mariño, Guillermo Megalou, Evgenia Morselli, Eugenia Rustin, Pierre Schroeder, Sabrina Shen, Shensi
Contributor
Bennetzen, Martin v. Bénit, Paule Cabrera, Sandra Criollo, Alfredo Eisenberg, Tobias Galluzzi, Lorenzo Horio, Yoshiyuki Kepp, Oliver Maiuri, Maria Chiara Malik, Shoaib Ahmad Mariño, Guillermo Megalou, Evgenia Morselli, Eugenia Rustin, Pierre Schroeder, Sabrina Shen, Shensi
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Description
Saccharomyces cerevisiae (WT BY4741-MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0) undergoing chronological aging on small synthetic 2% glucose media (SMD) without spermidine supplementation. Image represents EGFP-Atg8p localization, visualized by fluorescence microscopy. Yeast cells undergoing autophagy exhibit a prominent vacuolar localization of EGFP-Atg8p. Epifluorescence image was captured using a widefield microscope (Axioskop; Carl Zeiss, Inc) fitted with a Plan Neofluar 63× 1.25 NA oil immersion objective. Image acquired with a camera (SPOT 9.0 Monochrome 6; Diagnostic Instruments, Inc) using Metamorph software (Universal Imaging Corp) and processed with IrfanView and Photoshop (CS2; Adobe) software. Specifically, picture processing involved coloring and cropping of representative areas and was performed with IrfanView. In addition, linear adjustments of contrast and brightness were applied with Photoshop (using equal adjustment parameters for all pictures); no explicit γ correction was used. Image: Figure 2A, WT/Co/EGFP-Atg8p panel, in Morselli et al. J Cell Biol 192: 615-629 Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Morselli, Eugenia; Mariño, Guillermo; Bennetzen, Martin v.; Eisenberg, Tobias; Megalou, Evgenia; Schroeder, Sabrina; Cabrera, Sandra; Bénit, Paule; Rustin, Pierre; Criollo, Alfredo; Kepp, Oliver; Galluzzi, Lorenzo; Shen, Shensi; Malik, Shoaib Ahmad; Maiuri, Maria Chiara; Horio, Yoshiyuki (2021). CIL:13901, Saccharomyces cerevisiae S288c. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0PG1QDX
Type
image
Identifier
ark:/20775/bb11670522
Language
No linguistic content
Subject
BY 4741 Autophagy Autophagic vacuole Saccharomyces cerevisiae S288c Cell Image Library Group ID: 5406
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