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Description
The endosomal compartment is enlarged in the ema[1] mutant. Colocalization between DVGLUT (red) aggregates and the endosomal ESCRT protein Hrs (green, also known as Vps27) in motoneuron cell bodies of the ventral nerve cord of the ema[1] mutant. Wandering third instar larvae were dissected in ice-cold PBS and fixed with Bouin's solution for 5 min. Blocking and antibody incubation were performed in PBS containing 0.1% Triton X-100. anti-DVGLUT (Daniels et al., 2004) antibody was used at 1:10,000; anti-HRS-FL (Lloyd et al., 2002) was used at 1:1000. Secondary antibodies (Alexa 488-/Cy3-conjugated) were used at 1:1000. After staining, specimens were equilibrated in 70% glycerol in PBS and mounted with VectaShield (Vector Laboratories). Confocal images were acquired with a confocal microscope (model C1; Nikon) and accompanying EZ-C1 software using argon (excitation at 488 nm) and HeNe (excitation at 543 and 633 nm) lasers and a 60x Plan-Apochromat NA 1.4 objective (Nikon) at room temperature. Samples for each experiment were processed using the same confocal gain setting. Image corresponds to Figure 2A, bottom 3 panels in Kim et al. J Cell Biol. 188: 717-734. 2010. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Kim, Sungsu; Wairkar, Yogesh P.; Daniels, Richard W.; DiAntonio, Aaron (2021). CIL:13475, Drosophila melanogaster, motor neuron. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J06T0KCJ
Type
image
Identifier
ark:/20775/bb1201159q
Language
No linguistic content
Subject
Zinc ion binding Receptor-mediated endocytosis Ema[1] Endosome to lysosome transport Endosome transport Motor neuron Endosome Drosophila melanogaster Cell Image Library Group ID: 6022
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