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Description
Tem1-CAAX (green) is targeted away from spindle pole bodies in wild-type cells (CIL# 13880) but can also still localize to the SPB (this image). The CAAX motif from Ras2 targets proteins to the cytoplasmic side of the membrane. tem1Δ::GAL-UPL-TEM1 cells expressing eGFP-TEM1-CAAX from a CEN plasmid were grown on 2% raffinose/2% galactose and transferred to 2% glucose medium prior to image capture. A differential interference contrast (DIC) image is also shown. The tem1Δ::GAL1-UPL-TEM1 strain allows for the rapid, conditional depletion of Tem1. UPL, which stands for ubiquitin-proline-LacI, acts as a destabilizing module that permits rapid degradation of appended proteins. Image is Fig S5D, top panels, in J Cell Biol. (2011) 192: 599-614. Images in Fig S5 include CIL # 13880, 13881. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Valerio-Santiago, Mauricio; Monje-Casas, Fernando (2021). CIL:13881, Saccharomyces cerevisiae. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J00G3HVT
Type
image
Identifier
ark:/20775/bb2600511q
Language
No linguistic content
Subject
Small GTPase mediated signal transduction Regulation of exit from mitosis Mitosis Cell division W303 GTPase activity Protein binding Nucleotide binding Spindle pole body Saccharomyces cerevisiae Cell Image Library Group ID: 9034
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