Under copyright Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work. Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Rights Holder and Contact
UC Regents
Description
Expression of R98F PINK1-YFP (green) induces mitochondrial translocation of mCherry-Parkin (red) in the presence of the mitochondrial depolarizing agent CCCP (carbonyl cyanide-m-chlorophenyl hydrazone), indicating that this PINK1 mutant is functional for inducing Parkin translocation when located on the outer michondrial membrane. The R98F mutation in PINK1-YFP partially inhibits proteolytic cleavage. Transfected HeLa cells were treated with CCCP (10 µM) for 1 hr. Live cell imaging was performed on an LSM510 Meta (Carl Zeiss, Inc) with a 63x 1.4 NA oil differential interference contrast Plan Apo objective. Image contrast and brightness were adjusted in the LSM image browser (Zeiss). This image corresponds to Fig 4e, bottom row, with DMSO control in Fig 4e, top row of J Cell Biol, 191: 933-942, 2010. Images in Fig 4 include CIL#s 13733, 13734, 13729, 13730, 13731, 13732, 13717, 13718, 13719, 13720, 13721, 13722, 13723, 13724. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Jin, Seok Min; Lazarou, Michael; Wang, Chunxin; Kane, Lesley A.; Narendra, Derek P.; Youle, Richard J. (2021). CIL:13724, Homo sapiens, endothelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0ZP44VD J Cell Biol, 191: 933-942, 2010. https://www.ncbi.nlm.nih.gov/pubmed/?term=21115803
Type
Dataset
Language
No linguistic content; Not applicable
Subject
Protein binding ATP binding Response to stress Protein K48-linked ubiquitination Cervical carcinoma Ubiquitin-protein ligase activity Zinc ion binding Mitochondrion degradation Ubiquitin protein ligase binding HeLa C3HC4-type RING finger domain binding Protein serine/threonine kinase activity Intracellular protein kinase cascade Protein phosphorylation Oxidative phosphorylation uncoupler activity Calcium-dependent protein kinase activity Protein K63-linked ubiquitination Protein ubiquitination involved in ubiquitin-dependent protein catabolic process Magnesium ion binding Mitochondrion Mitochondrial outer membrane Cytosol Nucleus Mitochondrial inner membrane Endothelial cell Homo sapiens Cell Image Library Group ID: 7051
If you're wondering about permissions and what you can do with this item, a good starting point is the "rights information" on this page. See our terms of use for more tips.
Share your story
Has Calisphere helped you advance your research, complete a project, or find something meaningful? We'd love to hear about it; please send us a message.
