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Description
Tem1 (green) constitutively targeted to spindle pole bodies in nud1-2 cells at the restrictive temperature still displayed problems positioning the spindle (this image shows an aligned spindle, CIL #13889 shows a misaligned spindle and CIL# 13874 shows a rebudded cell) typical of nud1-2, despite the fact that viability was restored. Nud1 is required for mitotic exit and constitutive targeting of Tem1 recovered the mitotic exit defect of nud1-2 cells. DAPI (blue) and differential interference contrast (DIC) image are also shown. Image is Fig 7F, top panels, in J Cell Biol. (2011) 192: 599-614. Images in Fig 7 include CIL #13888, 13872, 13813, 13889, 13874. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Valerio-Santiago, Mauricio; Monje-Casas, Fernando (2021). CIL:13873, Saccharomyces cerevisiae. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J08G8JDS
Type
image
Identifier
ark:/20775/bb40339690
Language
No linguistic content
Subject
Protein binding Regulation of exit from mitosis GTPase activator activity Structural constituent of cytoskeleton Mitotic cell cycle spindle orientation checkpoint Mitosis Cell cycle Small GTPase mediated signal transduction W303 Exit from mitosis Nucleotide binding GTPase activity Cell division Cytoplasm Nuclear envelope Cytoskeleton Nucleus Spindle pole body Saccharomyces cerevisiae Cell Image Library Group ID: 9034
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