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Description
Intracellular localization of GFP-Vps74 in S. cerevisiae pik1-83 temperature-conditional strain grown at restrictive temperature of 37C. In pik1-83 mutant after a 30 minute incubation at the restrictive temperature, GFP-Vps74 loses punctate localization and is mostly in the cytosol and nucleus. The Golgi-localized PtdIns 4-kinase in yeast is composed of the Pik1 catalytic subunit and a myristoylated calcium-binding protein, Frq1. Vps74 is essential to maintain glycosyltransferases in the Golgi and results in this study reveal a previously unrecognized PtdIns4P-binding site in Vps74/GOLPH3 family proteins and link Pik1 signaling to retention of Golgi-resident proteins. Cells grown in liquid medium were mounted in growth medium and 3D image stacks were collected at 0.4-µm z increments on a DeltaVision workstation (Applied Precision) based on an inverted microscope (IX-70; Olympus) using a 100× NA 1.4 oil immersion lens. Images were captured at 23C with a 12-bit CCD camera (CoolSnap HQ; Photometrics) and deconvolved using the iterative-constrained algorithm (Agard, 1984) and the measured point spread function. One image from the approximate center of z stack is shown in Fig1A Vps74/pik1-83 37C panel in J Cell Biol. 187: 967-975. 2009. Images in Fig 1A include CIL#s 13439, 13440, 13441, 13442, 13443, 13444, 13445, 13446, 13447, 13448, 13458, 13459. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Wood, Christopher S.; Schmitz, Karl R.; Bessman, Nicholas J.; Setty, Thanuja Gangi; Ferguson, Kathryn M.; Burd, Christopher G. (2021). CIL:13459, Saccharomyces cerevisiae S288c. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J01J98FT J Cell Biol. 187: 967-975. 2009 https://www.ncbi.nlm.nih.gov/pubmed/?term=20026658
Type
image
Language
No linguistic content; Not applicable
Subject
Phosphatidylinositol-mediated signaling Phosphatidylinositol-4-phosphate binding Protein transport 1-phosphatidylinositol 4-kinase activity Enzyme binding Retrograde vesicle-mediated transport, Golgi to ER Pik1-83 Protein localization in Golgi apparatus Nucleus Trans-Golgi network Golgi apparatus Cytosol Extrinsic to membrane Saccharomyces cerevisiae S288c Cell Image Library Group ID: 6596
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