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Moving Image / CIL:13401, Strongylocentrotus purpuratus

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Title
CIL:13401, Strongylocentrotus purpuratus
Creator
Bement, William M
Miller, Ann L
Sider, Jenny R
Verbrugghe, Koen J.C
Von Dassow, George
Contributor
Bement, William M.
Miller, Ann L.
Sider, Jenny R.
Verbrugghe, Koen J.C.
Von Dassow, George
Date Created and/or Issued
2021
Contributing Institution
UC San Diego, Research Data Curation Program
Collection
Cell Image Library
Rights Information
Under copyright
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Rights Holder and Contact
UC Regents
Description
Eight-cell purple urchin embryo expressing three tandem GFPs fused to the ensconsin microtubule-binding domain (EMTB-3G; Faire et al., 1999). The embryo was treated with 20 µM nocodazole at time 00:00. Within 2 min, most astral microtubules disassembled; remaining microtubules are randomly oriented and most end well short of the cortex. Even so, furrowing is accurate and timely. Purple urchin were imaged with a laser scanning confocal microscope (Radiance 2000; Bio-Rad Laboratories) mounted on a compound microscope (E800; Nikon) using a 40× 1.3 NA Plan-Fluor oil lens. Room temperature was maintained at 14–17°C, which is tolerated well. Corresponds to Fig 3A and first segment of video 3 in J Cell Biol. 2009 Dec 14;187(6):831-45.
Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
Von Dassow, George; Verbrugghe, Koen J.C.; Miller, Ann L.; Sider, Jenny R.; Bement, William M. (2021). CIL:13401, Strongylocentrotus purpuratus. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J04B3008
Type
moving image
Identifier
ark:/20775/bb46482816
Language
No linguistic content
Subject
Nocodazole treatment
Embryo
Cytokinesis after mitosis
Mitotic anaphase
Cleavage furrow
Microtubule
Astral microtubule
Cell cortex
Ensconsin
Strongylocentrotus purpuratus
Cell Image Library Group ID: 6184

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