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Description
Macrophage cell line cell infected with Leishmania mexicana. Five amastigotes can be seen within a single large parasitophorous vacuole in the macrophage. Raw 264.7 cells (a leukaemic mouse macrophage line) were infected with amastigote form Leishmania mexicana (WHO strain MNYC/BZ/62/M379) at an MOI of 10 and maintained in RPMI for 4 days. Cells were fixed with 2% paraformaldehyde, and DNA was labelled with propidium iodide following incubation with 50 ug/ml RNaseA for 30 min at room temperature. Z stack of 93 images with a z spacing of 0.194 um captured with a 100x/1.4 NA objective lens on a DM5500 B epifluorescence microscope (Leica) with an Orca cooled CCD camera (Hamamatsu). Note there is a phase channel image that is not currently displayed, but can be turned on in the full viewer mode. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Wheeler, Richard (2021). CIL:10602, Leishmania mexicana, Mus musculus, macrophage, parasite. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J00Z720K
Type
image
Identifier
ark:/20775/bb50236750
Language
No linguistic content
Subject
WHO strain MNYC/BZ/62/M379 Amastigote form Leishmania mexicana Parasite entry into host cell forming a parasitophorous vacuole RAW264.7 Parasite-containing vacuole Parasite Macrophage Mus musculus Leishmania mexicana Cell Image Library Group ID: 3667
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