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Movie showing kinetochore alignment during mitosis in Drosophila S2 cells expressing GFP-Ndc80 to label kinetochores. One pair of chromatids becomes detached from the spindle, and anaphase is delayed until the pair is recaptured. Images were recorded at 60 sec intervals using a spinning disc confocal microscope on a Nikon TE-200 stand with a 100x 1.4 NA Plan APO DIC objective lens and recorded with a Hamamatsu Orca-ER CCD. See Maresca and Salmon, 2009 J Cell Biol 184:373-281. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Maresca, Thomas; Salmon, Edward (2021). CIL:25307, Drosophila melanogaster. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0G15ZRG Maresca and Salmon, 2009 J Cell Biol 184:373-281 https://www.ncbi.nlm.nih.gov/pubmed/?term=19193623