Under copyright Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work. Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Rights Holder and Contact
UC Regents
Description
Dual-channel spinning-disk confocal time-lapse movie showing anaphase relative to poleward microtubule flux. Fluorescent speckle microscopy (FSM) of microtubules was performed as described in Maddox et al. (2002, 2003), and with a 100X/1.4 NA Plan Apochromat objective (Nikon) with 2X2 binning in the cooled CCD camera (model ER; Hamamatsu Corporation). MetaMorph® software (Universal Imaging Corp.) was used to control shutters, wavelength selection, image acquisition, and storage. DNA was visualized by adding Oli-green to the extract (final concentration of 1 µg/ml). Images were acquired at 10-s intervals. Sequential images of different fluorophores were acquired at 3-15s intervals, depending on the experiment. Paired images from different color channels were within 0.5 s of each other. Movie is video 1 from J Cell Biol 2003 162:377-382. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Maddox, Paul S.; Straight, Aaron; Coughlin, Peg; Mitchison, Timothy J.; Salmon, Edward D. (2021). CIL:13088, Xenopus [NCBITaxon:262014]. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0028Q6J
If you're wondering about permissions and what you can do with this item, a good starting point is the "rights information" on this page. See our terms of use for more tips.
Share your story
Has Calisphere helped you advance your research, complete a project, or find something meaningful? We'd love to hear about it; please send us a message.
