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Description
Tem1 (green) normally localizes preferentially to the spindle pole body (SPB) that enters the daughter cell during anaphase; however, Tem1's ability to localize to the SPB is severely decreased in bfa1Δ cells (this image). tem1Δ::GAL-UPL-TEM1 cells expressing eGFP-TEM1 from a CEN plasmid were grown on 2% raffinose/2% galactose and transferred to 2% glucose medium prior to image capture. A differential interference contrast (DIC) image is also shown (hidden). The tem1Δ::GAL1-UPL-TEM1 strain allows for the rapid, conditional depletion of Tem1. UPL, which stands for ubiquitin-proline-LacI, acts as a destabilizing module that permits rapid degradation of appended proteins. Image is Fig 7A, top panels, in J Cell Biol. (2011) 192: 599-614. Images in Fig 7 include CIL #13888, 13872, 13813, 13889, 13874. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Valerio-Santiago, Mauricio; Monje-Casas, Fernando (2021). CIL:13888, Saccharomyces cerevisiae. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0PV6J4Z
Type
image
Identifier
ark:/20775/bb5126133c
Language
No linguistic content
Subject
Cell division Cell cycle Mitosis W303 Mitotic cell cycle spindle orientation checkpoint Small GTPase mediated signal transduction GTPase activator activity Protein binding GTPase activity Regulation of exit from mitosis Nucleotide binding Spindle pole body Cytoplasm Cytoskeleton Saccharomyces cerevisiae Cell Image Library Group ID: 9034
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