Under copyright Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work. Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Rights Holder and Contact
UC Regents
Description
Publication abstract: Ice nucleating particles (INPs) are a rare subset of aerosol particles that initiate cloud droplet freezing at temperatures above the homogenous freezing point of water (-38 °C). Considering that the ocean covers 70% of the earth’s surface and represent a large potential source of INPs, it is imperative that the uncertainties in the identities and emissions of ocean INP become better understood. However, the specific underlying drivers of marine INP emissions and their identities remain largely unknown due to limited observations and the challenge involved in isolating exceptionally rare IN forming particles. By generating nascent sea spray aerosol (SSA) over a range of biological conditions, mesocosm studies show that microbes can contribute to marine INPs. Here, we identify 14 (30%) cultivable halotolerant ice nucleating microbes and fungi among 47 total isolates recovered from precipitation and aerosol samples collected in coastal air in Southern California. IN isolates collected in coastal air were found to nucleate ice from extremely warm to moderate freezing temperatures (-2.3 to -18 °C). Air mass trajectory analyses, and cultivability in marine growth media indicate marine origins of these isolates. Further phylogenetic analysis confirmed that at least two of the 14 IN isolates were of marine origin. Moreover, results from cell washing experiments demonstrate that most IN isolates maintained freezing activity in the absence of nutrients and cell growth media. This study provides confirmation of previous studies’ findings that implicated microbes as a potential source of marine INPs and additionally demonstrates links between precipitation, marine aerosol and IN microbes. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Beall, Charlotte, M.; Michaud, Jennifer, M.; Fish, Meredith, A.; Dinasquet, Julie; Cornwell, Gavin, C.; Stokes, M., Dale; Burkart, Michael, D.; Hill, Thomas, C.; DeMott, Paul, J.; Prather, Kimberly, A. (2020). Data from: Cultivable, halotolerant ice nucleating bacteria and fungi in coastal precipitation. In Center for Aerosol Impacts on Chemistry of the Environment (CAICE). UC San Diego Library Digital Collections. https://doi.org/10.6075/J0GQ6W2Z This package contains an explanatory readme file and the data used to generate "Cultivable, halotolerant ice nucleating bacteria and fungi in coastal precipitation."
If you're wondering about permissions and what you can do with this item, a good starting point is the "rights information" on this page. See our terms of use for more tips.
Share your story
Has Calisphere helped you advance your research, complete a project, or find something meaningful? We'd love to hear about it; please send us a message.
