Image / CIL:12071, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist

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Title: CIL:12071, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist
Creator: Allen, Richard
Contributor: Allen, Richard
Date Created and/or Issued: 2021
Contributing Institution: UC San Diego, Research Data Curation Program
Collection: Cell Image Library
Rights Information: Under copyright
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Rights Holder and Contact: UC Regents
Description: This high resolution EM features the striated bands that link the membranes under the surface depressions of the cortex. The bands insert into the epiplasm, and in this case, they separate to pass around the parasomal sacs which lie to the right of a row of basal body/cilium complexes. The bands have relatively electron transparent zones next to the epiplasm that lie next to more opaque zones which are in turn separated by a central medium electron transparent zone. This differential banding suggests an overlap of filaments in the two more opaque zones, but there is no evidence of a muscle-like actomyosin system. These bands probably provide tension and keep the cortical ridges from flattening. If contractile they could account for the cell's ability to alter its shape when swimming through constricted regions. For details on the striated bands in Paramecium see Allen, J. Cell Biol. 49:1-20, 1971 and Allen, Aihara and Fok, J. Euk . Microbiol. 45:202-209, 1998. This image is adapted with permission from Fig. 14 in J. Cell Biol. 49:1-20, 1971. TEM taken on 12/18/68 by R. Allen with Philips 300 operating at 60kV. Neg. 16,000X. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
Allen, Richard (2021). CIL:12071, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J02N510X
Type: image
Identifier: ark:/20775/bb5979323p
Language: No linguistic content
Subject: Plasma membrane organization
Cortical cytoskeleton organization
Ciliated Protist
Eukaryotic cell
Microtubule basal body
Cell cortex
Cell by organism
Cilium
Eukaryotic Protist
Cortical striated fiber
Cortical cytoskeleton
Paramecium multimicronucleatum
Cell Image Library Group ID: 4846

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