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Description
Tem1 normally localizes preferentially to the spindle pole body (SPB) that enters the daughter cell during anaphase (this image and CIL# 13882). This image shows SPB localization of eGFP-Tem1 (eGFP, green) in anaphase cells. tem1Δ::GAL-UPL-TEM1 cells expressing eGFP-TEM1 from a CEN plasmid were grown on 2% raffinose/2% galactose and transferred to 2% glucose medium prior to image capture. Nuclear morphology was assessed by DAPI (blue). A differential interference contrast (DIC) image is also shown (gray). The tem1Δ::GAL1-UPL-TEM1 strain allows for the rapid, conditional depletion of Tem1. UPL, which stands for ubiquitin-proline-LacI, acts as a destabilizing module that permits rapid degradation of appended proteins. Image is Fig 1B, top panels, in J Cell Biol. (2011) 192: 599-614. Other images in Fig 1 include CIL #13882, 13883, 13884, 13885, 13886, 13887. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Valerio-Santiago, Mauricio; Monje-Casas, Fernando (2021). CIL:13885, Saccharomyces cerevisiae. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0610Z1P
Type
image
Identifier
ark:/20775/bb6900888b
Language
No linguistic content
Subject
Small GTPase mediated signal transduction Regulation of exit from mitosis Cell division W303 Mitosis GTPase activity Protein binding Nucleotide binding Spindle pole body Nucleus Saccharomyces cerevisiae Cell Image Library Group ID: 9034
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