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Localization and intracellular motility of myosin IIB (MIIB) in protrusive regions of migrating CHO.K1 cells. This Video corresponds to Fig. 6 B and video 4 in JCB 183:543-554 , 2008. Images of GFP-MIIB (green) and RFP-actin (magenta) were collected every 4 s using TIRF microscopy with 10 frames/s shown. Images were acquired in an inverted microscope (model IX70;Olympus). GFP and RFP were excited using the 488-nm laser line of an Ar ion laser and the 543-nm laser line of a He-Ne laser (Melles Griot), respectively. For dual GFP- mRFP/mCherry/mOrange, a dual-emission filter (z488/543) was used. Images were acquired with a charge-coupled device camera (Retiga Exi; Qimaging) and analyzed using MetaMorph software (MDS Analytical Technologies). Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Vicente-Manzanares, Miguel; Koach, Margaret A.; Whitmore, Leanna; Lamers, Marcelo L. (2021). CIL:12411, Cricetulus griseus, epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0C53JKZ
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