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The C2C12 cell line, a mouse myoblast line, was used here to study the regulatory factors in myogenic differentiation. After cultured in differentiation medium for 3 days, these cells differentiated into myotubes (Green) containing multiple nuclei (Blue). The C2C12 cells stably expressing FLAG-tagged rapamycin-resistant, kinase-inactive (RR/KI) mTOR were induced to differentiate in the presence of 50nM rapamycin (Rap) for 3 days. 25nM TSA was added when the cells were ~60% confluent and removed the next day upon induction of differentiation. TSA was able to rescue the effect of Rap to allow myotube maturation. This image is the control of different treatments in Figure 9B from JCB 189: 1157-1169, 2010. See also CIL: 13592, 13594, 13601. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Sun, Yuting; Ge, Yejing; Drnevich, Jenny; Zhao, Yong; Band, Mark; Chen, Jie (2021). CIL:13593, Mus musculus, myoblast. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J09885RZ
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