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Description
Microtubules in a Rac1(Q61L)-expressing PtK1 cell injected with Pak inhibitory fragment PBD/ID(H83L). Microtubules do not exhibit retrograde flow and undergo very little net growth. Epifluorescence image series were acquired on an inverted microscope (model TE300; Nikon) equipped with electronically controlled shutters, filter wheels, and a 14-bit cooled CCD camera (Orca II; Hamamatsu Corporation) controlled by MetaMorph® software (Universal Imaging Corp.) at 5-s intervals using a 60/1.4 NA Plan Apo objective lens (Nikon). Images were taken every 5 s and are played back at 15 frames/s. For still images, see Fig. 4, B and D of associated paper (J Cell Biol, 161:845-851, 2003). Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Wittmann, Torsten; Bokoch, Gary M.; Waterman-Storer, Clare M. (2021). CIL:11841, Potorous tridactylus, epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J09P30C6
Type
moving image
Identifier
ark:/20775/bb74469070
Language
No linguistic content
Subject
Activated Rac PAK inhibition Pt K1 (NBL-3) Microtubule polymerization Microtubule Epithelial cell Potorous tridactylus Cell Image Library Group ID: 4105
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