Under copyright Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work. Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Rights Holder and Contact
UC Regents
Description
HeLa cells expressing a kinetochore-specific protein were synchronized and released into S phase. At time intervals of 1,3,4,6,and 8 hr, cells were pulsed with BrdU, fixed and processed to label contromeres (red) and replicating DNA (green). Nuclear DNA was stained with DAPI (blue). Z-series image stacks were obtained using widefield microscopy, and the out-of-focus signal removed by constrained iterative deconvolution. Each column shows a single nucleus representative of the S-phase stages I - V. The top row (a-e) shows a merge of the red, green and blue signals, and the center row (f-j), the red and green signals only. The red and green signals were processed to reveal centromere-associated DNA replication which peaks in the latter half of S. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Shelby, Richard; Monier, Karine; Sullivan, Kevin (2021). CIL:39333, Homo sapiens, epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0ZW1JM5
Type
image
Identifier
ark:/20775/bb7617786d
Language
No linguistic content
Subject
HeLa DNA metabolic process DNA replication Condensed nuclear chromosome, centromeric region Nucleus Epithelial cell Homo sapiens
If you're wondering about permissions and what you can do with this item, a good starting point is the "rights information" on this page. See our terms of use for more tips.
Share your story
Has Calisphere helped you advance your research, complete a project, or find something meaningful? We'd love to hear about it; please send us a message.
