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Description
Expression of R98F PINK1-YFP (green), consistent with localization within mitochondria, does not induce mitochondrial translocation of mCherry-Parkin (red). The R98F mutation in PINK1-YFP partially inhibits proteolytic cleavage. Transfected HeLa cells were treated with DMSO for 1 hr. Live cell imaging was performed on an LSM510 Meta (Carl Zeiss, Inc) with a 63x 1.4 NA oil differential interference contrast Plan Apo objective. Image contrast and brightness were adjusted in the LSM image browser (Zeiss). This image corresponds to Fig 4e, top row, and is a control for Fig 4e, bottom row of J Cell Biol, 191: 933-942, 2010. Images in Fig 4 include CIL#s 13733, 13734, 13729, 13730, 13731, 13732, 13717, 13718, 13719, 13720, 13721, 13722, 13723, 13724. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Jin, Seok Min; Lazarou, Michael; Wang, Chunxin; Kane, Lesley A.; Narendra, Derek P.; Youle, Richard J. (2021). CIL:13723, Homo sapiens, endothelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0445K74
Type
image
Identifier
ark:/20775/bb7685875p
Language
No linguistic content
Subject
ATP binding Protein K48-linked ubiquitination Protein K63-linked ubiquitination Response to stress Cervical carcinoma Calcium-dependent protein kinase activity Intracellular protein kinase cascade Magnesium ion binding Ubiquitin-protein ligase activity Protein ubiquitination involved in ubiquitin-dependent protein catabolic process Mitochondrion degradation Zinc ion binding Protein phosphorylation HeLa Protein serine/threonine kinase activity Protein binding Ubiquitin protein ligase binding C3HC4-type RING finger domain binding Mitochondrial inner membrane Mitochondrial outer membrane Nucleus Mitochondrion Cytosol Endothelial cell Homo sapiens Cell Image Library Group ID: 7051
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