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Description
Tem1-CAAX (green) is targeted away from spindle pole bodies in bfa1Δ cells. The CAAX motif from Ras2 targets proteins to the cytoplasmic side of the membrane. tem1Δ::GAL-UPL-TEM1 cells expressing eGFP-TEM1-CAAX from a CEN plasmid were grown on 2% raffinose/2% galactose and transferred to 2% glucose medium prior to image capture. A differential interference contrast (DIC) image is also shown (hidden). The tem1Δ::GAL1-UPL-TEM1 strain allows for the rapid, conditional depletion of Tem1. UPL, which stands for ubiquitin-proline-LacI, acts as a destabilizing module that permits rapid degradation of appended proteins. Image is Fig 7A, bottom panels, in J Cell Biol. (2011) 192: 599-614. Images in Fig 7 include CIL #13888, 13872, 13813, 13889, 13874. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Valerio-Santiago, Mauricio; Monje-Casas, Fernando (2021). CIL:13872, Saccharomyces cerevisiae. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0251GX2
Type
image
Identifier
ark:/20775/bb7958918x
Language
No linguistic content
Subject
Nucleotide binding Cell cycle Regulation of exit from mitosis Cell division GTPase activator activity Protein binding W303 Small GTPase mediated signal transduction Mitosis Mitotic cell cycle spindle orientation checkpoint GTPase activity Cytoplasm Cytoskeleton Spindle pole body Saccharomyces cerevisiae Cell Image Library Group ID: 9034
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