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Moving Image / CIL:11985, Drosophila melanogaster, epithelial cell

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CIL:11985, Drosophila melanogaster, epithelial cell
Griffis, Eric R
Spudich, James A
Vale, Ronald D
Date Created and/or Issued
Contributing Institution
UC San Diego, Research Data Curation Program
Cell Image Library
Rights Information
Under copyright
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
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UC Regents
Time lapse of mCherry-tubulin by wide-field epifluorescence at the coverslip interface (left), mCherry-tubulin by TIRF (middle), and myosin-GFP by TIRF (right). The acquisition of Cherry-tubulin by TIRF was at 2s intervals, whereas the mCherry-tubulin by epifluorescence and myosin-GFP TIRF were at 36 and 10 s intervals, respectively. Video corresponds to Fig 3A and video 2 from J Cell Bio 186:727-738, 2009. Note that this video is the wild type control for another video in the series that shows the same biological process in the presence of kinesin-6 RNAi. Total internal reflection microscopy was performed using a microscope (Perfect-focus TE2000; Nikon) with a 100×, 1.45 NA objective (Nikon) and illumination from either a 488-nm argon laser (100 mW) or a 491-nm solid-state laser (100 mW) and a 561-nm solid-state laser (50 mW). For dual-color TIRF microscopy, we used a triplepass dichroic filter (z491/561/633rpc) and changed the emission filter (ET525/50 or ET595/50; both from Chroma Technology Corp.) with a filter wheel placed before the camera. In some cases, an excitation notch filter was used in the filter cube (NF01-405/488/561/635; Semrock, Inc.). Images were typically captured every 2-3 s with a 50-200 ms exposure with an EM charge-coupled device camera (iXon; Andor Technology). The microscope was controlled and images were acquired using open source MicroManager software (
Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (
Vale, Ronald D.; Spudich, James A.; Griffis, Eric R. (2021). CIL:11985, Drosophila melanogaster, epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset.
J Cell Bio 186:727-738, 2009
moving image
No linguistic content; Not applicable
Mitotic anaphase
Epithelial cell
Myosin regulatory light chain
Drosophila melanogaster
Cell Image Library Group ID: 3733

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