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Description
Time-lapse of Toxoplasma gondii. A monolayer of human foreskin fibroblast cells was infected with Toxoplasma gondii expressing EGFP-tagged TgIMC1 and mRFP-tagged TgMORN1 (MORN domain containing protein). Formation of daughter cells is visible during time-lapse. Images acquired using API DeltaVision on a Olympus IX70 inverted microscope. Objective: Olympus 60X water immersion, 1.2 NA. Filters: EGFP, ex 450-490 em 500-540, mRFP, ex 555-592 em 600-665. Dichromatic mirror: Chroma 89021. Captured with CoolSnapHQ/ICX285. 3D stacks of images were collected at 0.6 μ increments in z, every 10 min. The epifluorescence image is a maximum intensity projection of the deconvolved 3D stack at each timepoint. Corresponding raw data in CIL# 10522. Corresponding deconvolved data in CIL# 10523. Corresponding movie: CIL# 10525. Unpublished image that is similar to images published in PMID: 16518471 and 20140195. See publications for other experimental details. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Murray, John; Hu, Ke (2021). CIL:10524, Toxoplasma gondii RH. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0Z60N6R
Type
image
Identifier
ark:/20775/bb83348702
Language
No linguistic content
Subject
Cytoskeleton organization Cell division Toxoplasma gondii RH stable cell line Toxoplasmosis Regulation of cell shape Basal part of cell Spindle pole Centriole Inner membrane complex Conoid Toxoplasma gondii RH Cell Image Library Group ID: 5774
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