Image / CIL:20803, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist

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Title: CIL:20803, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist
Creator: Allen, Richard
Contributor: Allen, Richard
Date Created and/or Issued: 2021
Contributing Institution: UC San Diego, Research Data Curation Program
Collection: Cell Image Library
Rights Information: Under copyright
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Rights Holder and Contact: UC Regents
Description: To determine what happens to the CV during systole we observed the CV in a living cell and then fixed the cell as soon as the CV had opened to the outside. It was apparent that the CV membrane does not flatten into an empty sac but the CV membrane undergoes rapid tubule formation and collapses as a continuous mat of tubules that maintains its connection to the microtubular ribbons. These tubules are a uniform 40nm in diameter. TEM taken on 6/9/96 by R. Allen with Zeiss 10A operating at 80kV. Neg. 19,800X. Bar = 0.2µm. Adapted with permission from the J. Exp. Biol. 200:1737-1744, 1997. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. An unprocessed, high resolution version of this image (CIL:13113) is in the library and available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
Allen, Richard (2021). CIL:20803, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J01J98G8
Type: image
Identifier: ark:/20775/bb8539143s
Language: No linguistic content
Subject: Cytoplasmic microtubule organization
Contractile vacuole organization
Integral to contractile vacuolar membrane
Cell by organism
Contractile vacuolar membrane
Eukaryotic cell
Intrinsic to contractile vacuolar membrane
Eukaryotic Protist
Ciliated Protist
Cytoplasmic microtubule
Paramecium multimicronucleatum
Cell Image Library Group ID: 5664

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