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The image shows a 3D-rendering (Imaris software) of a live confluent culture of NIH-3T3 cells obtained using confocal microscopy. The cells were co-transduced with 5 fluorescent proteins and with Lentiviral Gene Ontology (LeGO) vectors expressing Cerulean (blue), EGFP (green), Venus (yellow), tdTomato (magenta) or mCherry (red) fluorescent proteins to provide combinatorial colors for progeny tracking. Groups of nearby cells of the same color descended from same stem cells. This image is grouped with two others obtained using a similar imaging strategy. See also Malide et al. (2012) Dynamic clonal analysis of murine hematopoietic stem and progenitor cells marked by 5 fluorescent proteins using confocal and multiphoton microscopy. Blood. Dec 20;120(26):e105-16. An image obtained in the same manner that obtained an Honorable Mention in the 2011 Olympus BioScapes Digital Imaging Competition may be viewed as CIL:41631. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Malide, Daniela; Metais, Jean-Yves; Dunbar, Cynthia (2021). CIL:44151, Mus musculus, fibroblast. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0PK0F1B
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