Bennetzen, Martin v Bénit, Paule Cabrera, Sandra Criollo, Alfredo Eisenberg, Tobias Galluzzi, Lorenzo Horio, Yoshiyuki Kepp, Oliver Maiuri, Maria Chiara Malik, Shoaib Ahmad Mariño, Guillermo Megalou, Evgenia Morselli, Eugenia Rustin, Pierre Schroeder, Sabrina Shen, Shensi
Contributor
Bennetzen, Martin v. Bénit, Paule Cabrera, Sandra Criollo, Alfredo Eisenberg, Tobias Galluzzi, Lorenzo Horio, Yoshiyuki Kepp, Oliver Maiuri, Maria Chiara Malik, Shoaib Ahmad Mariño, Guillermo Megalou, Evgenia Morselli, Eugenia Rustin, Pierre Schroeder, Sabrina Shen, Shensi
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Description
Human colon carcinoma HCT 116 cells were transfected with an siRNA specific for SIRT1 and then retransfected with a GFP-LC3-encoding plasmid, cultured in complete medium for 24 h and left untreated for 4 h as a control for resveratrol or spermidine treatment. For fluorescence microscopy determinations, cells cultured on coverslips were fixed in paraformaldehyde (4% wt/vol) for 15 min at RT, washed three times in PBS, and mounted with mounting medium (Vectashield). Confocal fluorescent images were captured using a confocal fluorescence microscope (TCS SP2; Leica) fitted with an Apochromat 63× 1.3 NA immersion objective. Images were acquired with a camera (DFC 350 FX 1.8.0; Leica) using LAS AF software (Leica) and processed with Photoshop (CS2; Adobe) software. Specifically, picture processing involved cropping of representative areas and linear adjustments of contrast and brightness and was performed using Photoshop (with equal adjustment parameters for all pictures); no explicit γ correction was used. Image: Figure 1A, bottom left panel, in Morselli et al. J Cell Biol 192: 615-629 Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Morselli, Eugenia; Mariño, Guillermo; Bennetzen, Martin v.; Eisenberg, Tobias; Megalou, Evgenia; Schroeder, Sabrina; Cabrera, Sandra; Bénit, Paule; Rustin, Pierre; Criollo, Alfredo; Kepp, Oliver; Galluzzi, Lorenzo; Shen, Shensi; Malik, Shoaib Ahmad; Maiuri, Maria Chiara; Horio, Yoshiyuki (2021). CIL:13906, Homo sapiens, permanent cell line cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0416VSZ
Type
image
Identifier
ark:/20775/bb8948693s
Language
No linguistic content
Subject
Autophagy HCT 116 Permanent cell line cell Autophagic vacuole Homo sapiens Cell Image Library Group ID: 5405
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