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Moving Image / CIL:15791, Dendraster excentricus

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CIL:15791, Dendraster excentricus
Bement, William M
Miller, Ann L
Sider, Jenny R
Verbrugghe, Koen J.C
Von Dassow, George
Date Created and/or Issued
Contributing Institution
UC San Diego, Research Data Curation Program
Cell Image Library
Rights Information
Under copyright
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
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UC Regents
One of eight cells in a slightly compressed sand dollar embryo expressing three tandem GFPs fused to the ensconsin microtubule-binding domain (EMTB-3G; Faire et al., 1999). The embryo was treated with 20 µM trichostatin A (TSA), an inhibitor of HDAC6 (a tubulin deacetylase) that selectively disrupts dynamic microtubules in mammalian cells at time 00:00, before nuclear envelope breakdown. After an exceptionally long metaphase (>20 min) with no long astral microtubules, and during which one half of the spindle bifurcated, this cell entered anaphase approximately 26 min after TSA addition. Some astral microtubules regrew in anaphase, but none approach within 10 µm of the cortex. Sand dollar embryos were imaged with a laser scanning confocal microscope (Radiance 2000; Bio-Rad Laboratories) mounted on a compound microscope (E800; Nikon) using a 40× 1.3 NA Plan-Fluor oil lens. Room temperature was maintained at 14–17°C. Video corresponds to the fourth segment of Video 4 and Figure S4B of J Cell Biol. 2009 Dec 14;187(6):831-45.
Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (
Von Dassow, George; Verbrugghe, Koen J.C.; Miller, Ann L.; Sider, Jenny R.; Bement, William M. (2021). CIL:15791, Dendraster excentricus. In Cell Image Library. UC San Diego Library Digital Collections. Dataset.
J Cell Biol. 2009 Dec 14;187(6):831-45.
moving image
No linguistic content; Not applicable
TSA treatment
Mitotic anaphase
Mitotic metaphase
Astral microtubule
Dendraster excentricus
Cell Image Library Group ID: 6184

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