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Image / CIL:11987, Drosophila melanogaster, epithelial cell

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Title
CIL:11987, Drosophila melanogaster, epithelial cell
Creator
Griffis, Eric R
Spudich, James A
Vale, Ronald D
Contributor
Griffis, Eric R.
Spudich, James A.
Vale, Ronald D.
Date Created and/or Issued
2021
Contributing Institution
UC San Diego, Research Data Curation Program
Collection
Cell Image Library
Rights Information
Under copyright
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Rights Holder and Contact
UC Regents
Description
TIRF time lapse of myosin-GFP (left), mCherry-tubulin (middle), and overlay (myosin-GFP in green and mCherry-tubulin in red) in wild-type S2 cells from metaphase to anaphase. This cell has many astral microtubules touching the cortex (within the TIRF illumination field) in the bottom left and very few in the top right. Despite this asymmetry (perhaps due to a tilting of the spindle), myosin localization occurs in a normal, symmetrical manner, and myosin clears from both poles. Video corresponds to video 4 from J Cell Bio 186:727-738, 2009. Total internal reflection microscopy was performed using a microscope (Perfect-focus TE2000; Nikon) with a 100×, 1.45 NA objective (Nikon) and illumination from either a 488-nm argon laser (100 mW) or a 491 nm solid-state laser (100 mW) and a 561-nm solid-state laser (50 mW). For dual-color TIRF microscopy, we used a triplepass dichroic filter (z491/561/633rpc) and changed the emission filter (ET525/50 or ET595/50; both from Chroma Technology Corp.) with a filter wheel placed before the camera. In some cases, an excitation notch filter was used in the filter cube (NF01-405/488/561/635; Semrock, Inc.). Images were typically captured every 2-3 s with a 50-200 ms exposure with an EM charge-coupled device camera (iXon; Andor Technology). The microscope was controlled and images were acquired using open source MicroManager software (http://www.micro-manager.org).
Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
Vale, Ronald D.; Spudich, James A.; Griffis, Eric R. (2021). CIL:11987, Drosophila melanogaster, epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0P55M89
Type
image
Identifier
ark:/20775/bb92899332
Language
No linguistic content
Subject
Mitotic anaphase
Mitotic metaphase
S2
Epithelial cell
Myosin regulatory light chain
Spindle
Drosophila melanogaster
Cell Image Library Group ID: 3733

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