Skip to main content

Dataset / CIL:11987, Drosophila melanogaster, epithelial cell

Have a question about this item?

Item information. View source record on contributor's website.

CIL:11987, Drosophila melanogaster, epithelial cell
Griffis, Eric R
Spudich, James A
Vale, Ronald D
Date Created and/or Issued
Contributing Institution
UC San Diego, Research Data Curation Program
Cell Image Library
Rights Information
Under copyright
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Rights Holder and Contact
UC Regents
TIRF time lapse of myosin-GFP (left), mCherry-tubulin (middle), and overlay (myosin-GFP in green and mCherry-tubulin in red) in wild-type S2 cells from metaphase to anaphase. This cell has many astral microtubules touching the cortex (within the TIRF illumination field) in the bottom left and very few in the top right. Despite this asymmetry (perhaps due to a tilting of the spindle), myosin localization occurs in a normal, symmetrical manner, and myosin clears from both poles. Video corresponds to video 4 from J Cell Bio 186:727-738, 2009. Total internal reflection microscopy was performed using a microscope (Perfect-focus TE2000; Nikon) with a 100×, 1.45 NA objective (Nikon) and illumination from either a 488-nm argon laser (100 mW) or a 491 nm solid-state laser (100 mW) and a 561-nm solid-state laser (50 mW). For dual-color TIRF microscopy, we used a triplepass dichroic filter (z491/561/633rpc) and changed the emission filter (ET525/50 or ET595/50; both from Chroma Technology Corp.) with a filter wheel placed before the camera. In some cases, an excitation notch filter was used in the filter cube (NF01-405/488/561/635; Semrock, Inc.). Images were typically captured every 2-3 s with a 50-200 ms exposure with an EM charge-coupled device camera (iXon; Andor Technology). The microscope was controlled and images were acquired using open source MicroManager software (
Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (
Vale, Ronald D.; Spudich, James A.; Griffis, Eric R. (2021). CIL:11987, Drosophila melanogaster, epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset.
J Cell Bio 186:727-738, 2009
No linguistic content; Not applicable
Mitotic anaphase
Mitotic metaphase
Epithelial cell
Myosin regulatory light chain
Drosophila melanogaster
Cell Image Library Group ID: 3733

About the collections in Calisphere

Learn more about the collections in Calisphere. View our statement on digital primary resources.

Copyright, permissions, and use

If you're wondering about permissions and what you can do with this item, a good starting point is the "rights information" on this page. See our terms of use for more tips.

Share your story

Has Calisphere helped you advance your research, complete a project, or find something meaningful? We'd love to hear about it; please send us a message.

Explore related content on Calisphere: