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Image / CIL:23045, Rattus, fibroblast

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Title
CIL:23045, Rattus, fibroblast
Creator
Borisy, Gary G
Svitkina, Tatyana M
Verkhovsky, Alexander B
Contributor
Borisy, Gary G.
Svitkina, Tatyana M.
Verkhovsky, Alexander B.
Date Created and/or Issued
2021
Contributing Institution
UC San Diego, Research Data Curation Program
Collection
Cell Image Library
Rights Information
Under copyright
Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work.
Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Rights Holder and Contact
UC Regents
Description
Preferential distribution of plectin in peripheral regions. REF-52 cells were treated with nocodazole to depolymerize MTs and induce collapse of most IFs to the cell center. Electron microscopy of peripheral cell regions after gelsolin treatment and immunogold (10 nm) labeling for plectin. Short intermediate filaments at the periphery have numerous plectin sidearms. Electron microscopy of cytoskeletons was performed as described (Svitkina et al., 1995). Briefly, cells on coverslips were lysed as for light microscopy, treated, with recombinant gelsolin NHz-terminal domain, fixed with glutaraldehyde, tannic acid and uranyl acetate, critical point dried, and coated with platinum and carbon. Image corresponds to Fig 1c in J Cell Biol. 1996 Nov;135(4):991-1007.
Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
Svitkina, Tatyana M.; Verkhovsky, Alexander B.; Borisy, Gary G. (2021). CIL:23045, Rattus, fibroblast. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J04T6H7K
Type
image
Identifier
ark:/20775/bb9290015r
Language
No linguistic content
Subject
REF-52
Gelsolin treatment
Nocodazole treatment
Intermediate filament
Plectin
Fibroblast
Rattus
Cell Image Library Group ID: 8924

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