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Description
The seeds of Pinot noir (Pn) and Cabernet Sauvignon (CS) were analyzed by HPLC for monomeric and polymeric polyphenol ( tannin) content. The Pn and CS seeds had similar concentrations of polymeric polyphenols, but the Pn seeds had an order of magnitude higher concentration of the monomeric flavan-3-ols. Pn and CS wines were fermented with and without the addition of total grape seed tannin. The Pn control wine contained no polymeric polyphenols, while the tannin-added treatment wine contained polymeric material which was not bound to anthocyanin as determined by absorbance at 520 nm. Instead, the 520 nm absorbance in the Pn wine was confined to a broad peak in the non-polymeric region. Both treatment and control CS wines contained non-polymeric and polymeric fractions with absorbance at 520 nm. We hypothesize that this is due to competitive binding to the anthocyanins of the monomers in preference to the polymers in Pn.
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