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Description
Local application of calyculin A (phosphatase inhibitor) can stimulate motility initiation. Phase-contrast time-lapse video of a stationary keratocyte exposed to a local application of calyculin A. Red pseudocolor indicates the calyculin A flow from the micropipette (positioned in the bottom left corner). The keratocyte polarized and initiated motility away from the source of calyculin A. 50x real time (frames were collected at 5-s intervals and are displayed at 10 frames/s). Cells were imaged in culture media (Leibovitz's L-15 medium without phenol red supplemented with 14.2 mM Hepes, pH 7.4, 10% FBS, and 1% antibiotic-antimycotic). Corresponds to Fig. 6 C and video 7 in JCB 178:1207-1221, 2007. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Yam, Patricia T.; Wilson, Cyrus A.; Ji, Lin; Hebert, Benedict; Barnhar, Erin L.; Dye, Natalie A.; Wiseman, Paul W.; Danuser, Gaudenz; Theriot, Julie (2021). CIL:12016, Hypsophrys nicaraguensis, keratocyte. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0H41Q5S
Type
moving image
Identifier
ark:/20775/bb0006572x
Language
No linguistic content
Subject
Negative regulation of phosphatase activity Keratocyte Actin cytoskeleton Hypsophrys nicaraguensis Cell Image Library Group ID: 3422
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