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Description
Fluorescence recovery after photobleaching (FRAP)kinetics of MeCP2 were recorded using a mouse fibroblast cell line stably expressing MeCP2-GFP. Live cells were imaged using a Zeiss LSM 510 meta confocal microscope with fluorescence excitation at 488 nm and a 63x plan-apo oil lens. The image series has 3 pre-bleach shots, one immediately after bleaching a 1 micrometer diameter circle of pericentromeric heterochromatin, followed by 79 images at 2 sec intervals. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Ghosh, Rajarshi; Woodcock, Christopher (2021). CIL:8042, Mus musculus. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J09C6WGB
Type
image
Identifier
ark:/20775/bb00412056
Language
No linguistic content
Subject
Mobility of nuclear protein Nuclear chromatin Mus musculus Cell Image Library Group ID: 1515
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