Creative Commons Public Domain Dedication Constraint(s) on Use: This work may be used without prior permission. Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Description
Human umbilical vein endothelial cells (HUVECS) labeled for focal adhesions (green) and F-actin (red). HUVECs were plated on polyacrilamide gels (1.5 Kpa) coated with 1.0 mg/ml fibronectin. After 24 hrs cells were fixed in 3% paraformaldehyde and stained with mouse antibody to Phosphotyrosine (4G10) monoclonal antibody detected with goat anti-mouse Alexa 565 and Alexa 488 phalloidin to stain for F-actin. Image was collected on a Nikon Eclipse with a cooled charge coupled device (CCD; Orca II; Hamamatsu Photonics) using a 60x 1.4 NA Plan Apo PH objective lens (Nikon). The exposure time was 320 msec each channel. A xenon arc lamp was used with a Semrock DA/FI/TR/Cy5-4x4M-B Sedat filter set. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Pasapera, Ana M.; Waterman, Clare M. (2021). CIL:7440, Homo sapiens, primary cell line cell, endothelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0CN72WJ
Type
image
Identifier
ark:/20775/bb00753190
Language
No linguistic content
Subject
HUVEC Phosphotyrosine binding Regulation of focal adhesion assembly Primary cell line cell Focal adhesion Actin cytoskeleton Endothelial cell Homo sapiens
If you're wondering about permissions and what you can do with this item, a good starting point is the "rights information" on this page. See our terms of use for more tips.
Share your story
Has Calisphere helped you advance your research, complete a project, or find something meaningful? We'd love to hear about it; please send us a message.
