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Description
This ghoulish-looking Schwann cell was present in a primary culture of the dorsal root ganglion of mouse that was transfected with a mutant peripherin transgene. The intermediate filament network (vimentin antibody labeling in green) is collapsed around the nucleus 48 hr after transfection, and is correlated with a corresponding peri-nuclear clustering of the mitochondria (labeled with Mitotracker). Mitotracker CMX Ros was added to the culture medium for 30 mins, then rinsed out for 45 minutes in culture before fixing the cells with methanol. Microscope - Zeiss Axioplan I Illumination - Mercury arc lamp Objective - 100X oil/1.3 NA Intermediate lens between obj and camera - 0.63X Camera - Axiocam MRM CCD Filters: 1. Ex 485/20, Em 515-565 BP, dichroic 510 2. Ex 560/40, Em 630/60, dichroic 595 Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Parysek, Linda (2021). CIL:247, Mus musculus, myelinating Schwann cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0057F7B
Type
image
Identifier
ark:/20775/bb0109229c
Language
No linguistic content
Subject
Intermediate filament-based process Mitochondrion localization Cytoplasm organization ATP biosynthetic process Myelinating Schwann cell Type III intermediate filament Mitochondrion Mus musculus
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