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Image / CIL:12595, Trypanosoma brucei, cell by organism, eukaryotic cell, Eukaryotic Protist, Flagellated Protist

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Title
CIL:12595, Trypanosoma brucei, cell by organism, eukaryotic cell, Eukaryotic Protist, Flagellated Protist
Creator
Hoog, Johanna
Contributor
Hoog, Johanna
Date Created and/or Issued
2021
Contributing Institution
UC San Diego, Research Data Curation Program
Collection
Cell Image Library
Rights Information
Creative Commons Public Domain Dedication
Constraint(s) on Use: This work may be used without prior permission.
Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Description
The flagellar pocket of a Trypanosoma brucei bloodstream form cell, with short flagellum. The image shows a new flagellum starting to grow from the flagellar pocket, in preparation for the next cell division. Cells were high pressure frozen and freeze substituted (1hr freeze substitution in 2% uranyl acetate in acetone and 8% methanol). An 80nm thick section was stained for 5 minutes with uranyl acetate, one minute with lead citrate and viewed using a Philips CM100 transmission electron microscope. The image was captured with an Advantage HS-B camera (2000 x 2000 pixels; AMT, Danvers, MA, USA). Contrast adjusted using Photoshop. Magnification indicated by scale bar.
Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
Hoog, Johanna (2021). CIL:12595, Trypanosoma brucei, cell by organism, eukaryotic cell, Eukaryotic Protist, Flagellated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0QV3KMT
Type
image
Identifier
ark:/20775/bb0211952r
Language
No linguistic content
Subject
Cortical microtubule organization
Flagellar axoneme assembly
Ciliary or flagellar motility
Developing basal body
Cell by organism
Flagellated Protist
Axoneme
Axoneme part
Eukaryotic cell
Eukaryotic Protist
Cortical microtubule, transverse to long axis
Microtubule basal body
Trypanosoma brucei

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