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Description
Mitochondria are sequestered inside Rab5+ endosomes after FCCP treatment in WT and Atg5-/- MEFs. Wild-type (WT) and Atg5-/- mouse embryonic fibroblasts (MEFs) were transfected with GFP-Rab5, nanobody against GFP (VHH)-APEX2, HA-Parkin, and mPlum-mito-3 using Fugene 6. Cells were treated with DMSO or 25 uM FCCP for 4 hours and fixed with 2.5% gluteraldehyde. Cells were imaged by confocal microscopy and subsequently processed and imaged for transmission electron microscopy. Colocalizing puncta found by confocal microscopy, indicating mPlum-mito-3+ mitochondria within GFP-Rab5+ early endosomes, were correlated to their location in the electron micrographs. Tomograms were acquired of these locations, which morphological confirm the presence of mitochondria within single-membraned early endosomes. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Hammerling, Babette; Najor, Rita; Cortez, Melissa; Shires, Sarah; Leon, Leonardo; Gonzalez, Eileen; Boassa, Daniela; Phan, Sébastien; Thor, Andrea; Jimenez, Rebecca; Li, Hong; Kitsis, Richard; Dorn, Gerald; Sadoshima, Junichi; Ellisman, Mark; Gustafsson, Asa (2021). CIL:49418, Mus musculus, embryonic cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0WH2NKX
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