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Description
COS7 cells, transfected with GFP-PTP1B and APN-EphA3, when co-transfected with TM-BirA, yielded biotinylated EphA3 that can be stimulated with SA-Dynabeads. GFP-PTP1B and EphA3-transfected COS7 cells were stimulated with Alexa Fluor 594–ephrinA5–Fc–coated Protein A Dynabeads. Cell surface EphA3 was labelled on intact with anti-EphA3 mAb and detected with Alexa546-labelled secondary antibodies by confocal microscopy. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Cell Image Library (2021). CIL:36022, Chlorocebus aethiops, permanent cell line cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0K93664
Type
image
Identifier
ark:/20775/bb0313924h
Language
No linguistic content
Subject
Protein tyrosine kinase activity COS-7 Negative regulation of insulin receptor signaling pathway Permanent cell line cell Chlorocebus aethiops Cell Image Library Group ID: 8847
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