Creative Commons Public Domain Dedication Constraint(s) on Use: This work may be used without prior permission. Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Description
This is IMAGE #2 (10 minutes) of a time lapse series contained with in this image group. It shows neurons from the optic tectum of an albino Xenopus laevis tadpole CNS, stage 47. The images were acquired with 10 minute intervals and show morphological plasticity of the dendritic arbor with branch additions and retractions. These cells were transfected with in vivo electroporation of plasmid DNA encoding eYFP ~24 hours before the first image was acquired. The intact, living tadpole was anesthetized, positioned under a glass coverslip and this image was taken directly through its transparent skin using a custom built two-photon microscope. Images were acquired every 10 minutes for 1 hour. After 60 minutes, the animal was placed in a 50 microMolar anisomycin solution for ~1.5 hours. A second time lapse series (10 minute interval for 60 minutes) was then acquired. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Bestman, Jennifer; Cline, Holly (2022). CIL:36855, Xenopus laevis, CNS neuron (sensu Vertebrata). In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0NG4QDR
Type
image
Identifier
ark:/20775/bb0314486m
Language
No linguistic content
Subject
Morphogenesis of a branching structure Neuron development Dendrite Axon Cytoplasm Neuronal cell body CNS neuron (sensu Vertebrata) Xenopus laevis Cell Image Library Group ID: 9191
If you're wondering about permissions and what you can do with this item, a good starting point is the "rights information" on this page. See our terms of use for more tips.
Share your story
Has Calisphere helped you advance your research, complete a project, or find something meaningful? We'd love to hear about it; please send us a message.
