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Description
Amphibian oocytes have many copies of rRNA genes that are actively transcribed. Newt oocyte nuclei were dissected, allowed to swell in a low ionic strength high pH buffer, centrifuged through a sucrose-formaldehyde cushion onto EM grids coated with carbon films, and, in this iamge, stained with aqueous uranly acetate. The strand stretching from top left to bottom right contains two tree-like transcribed genes showing the closely packed RNA polymerases with inceasingly large nascent rRNA transcripts as they approach the termination point. Nascent transcripts are more compact with negative than positive staining. Nucleosomes are visible in the spacer region between transcripts. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Woodcock, Christopher (2021). CIL:825, Notophthalmus viridescens, oocyte. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0VM4BGB
Type
image
Identifier
ark:/20775/bb03822872
Language
No linguistic content
Subject
RNA metabolic process Ribosomal RNA transcription Nuclear chromosome Oocyte Notophthalmus viridescens Cell Image Library Group ID: 10245
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