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Description
Movie raw file of RPE-1 cells expressing GFP-alpha-tubulin and depleted for pVHL (Von Hippel-Lindau) by ectopic expression of shRNAmir against VHL. Video used to manually track MTs and determine growth speed and shrinkage time. Cells on round coverslips were transferred to a homemade holding device, and 600 µl of the appropriate medium was added. The video was acquired with a microscope (IX70 Delta Vision Spectris; Olympus), temperature-controlled at 37C, using a 100× NA 1.4 differential interference contrast (DIC) oil Plan-Apochromat objective, ex 470 em 520, and a camera (CoolSNAP HQ; Roper Industries) with an exposure time of 200 ms and a frame rate of 2Hz. Acquisition software used was SoftWoRx version 3.3.4 (Applied Precision). This video corresponds to J Cell Biol. 2010. 190: 991-1003--Supplementary Figure 1. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Thoma, Claudio R.; Matov, Alexandre; Gutbrodt, Katrin L.; Hoerner, Christian R.; Smole, Zlatko; Krek, Wilhelm; Danuser, Gaudenz (2021). CIL:26573, Homo sapiens, epithelial cell, renal pigment epithelium. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J02V2DXH
Type
image
Identifier
ark:/20775/bb05527629
Language
No linguistic content
Subject
Protein polymerization RPE-1 Structural molecule activity GTPase activity Cytoskeleton Epithelial cell Renal pigment epithelium Protein complex Microtubule Homo sapiens Cell Image Library Group ID: 8073
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