Image / CIL:9856, Paramecium tetraurelia, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist

View source image on contributor's website.

Get Citation

Have a question about this item?

Contact Owner

Item information. View source record on contributor's website.

Title: CIL:9856, Paramecium tetraurelia, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist
Creator: Allen, Richard
Contributor: Allen, Richard
Date Created and/or Issued: 2021
Contributing Institution: UC San Diego, Research Data Curation Program
Collection: Cell Image Library
Rights Information: Creative Commons Public Domain Dedication
Constraint(s) on Use: This work may be used without prior permission.
Use: The person(s) who associated a work with this deed has dedicated the work to the public domain by waiving all of their rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law.
Description: In mitosis the nuclear envelope of the micronucleus becomes heavily and uniformly labeled with anti-b-tubulin which is not in the form of microtubules. Some tubulin is also present inside the micronucleus. Apparently b–tubulin is being recruited to the mitotic micronucleus to form the spindle inside the intact micronucleus. Microtubules are present in the cytosol. TEM taken on 7/5/96 by R. Allen with Zeiss 10A operating at 80kV. Neg. 7,900X. Bar = 1µm. Cells were lightly fixed with 0.25% glutaraldehyde and infiltrated with 2.3M sucrose before being frozen in liquid nitrogen and thin sectioned at a temperature of –100°C at approximately 75nm thickness. Frozen sections from these preparations were then thawed, washed, and exposed to a monoclonal primary antibody that was raised in mice or rabbit/goat and to colloidal gold-complexed goat-anti-mouse/rabbit secondary antibodies. Further details of preparation are detailed in Methods Cell Biol. 2010;96:143-73. The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. An unprocessed, high resolution version of this image (CIL:38688) is in the library and available for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp)
Allen, Richard (2021). CIL:9856, Paramecium tetraurelia, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0X929F7
Type: image
Identifier: ark:/20775/bb0553211j
Language: No linguistic content
Subject: Micronucleus organization
Recruitment of b-tubulin
Nuclear division
Eukaryotic Protist
Nuclear envelope
Cell by organism
Ciliated Protist
Eukaryotic cell
Micronucleus
B-tubulin
Paramecium tetraurelia
Cell Image Library Group ID: 9636

About the collections in Calisphere

Learn more about the collections in Calisphere. View our statement on digital primary resources.

Copyright, permissions, and use

If you're wondering about permissions and what you can do with this item, a good starting point is the "rights information" on this page. See our terms of use for more tips.

Share your story

Has Calisphere helped you advance your research, complete a project, or find something meaningful? We'd love to hear about it; please send us a message.