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Description
Time-lapse microscopy of a spreading MIIB (myosin II B)-deficient cell cell expressing RLC-D,D–GFP (a phospho-mimetic mutant of the RLC) on fibronectin. Arrows point to clusters, or proto-bundles, denoted by the accumulation of MIIA (the only isoform present in the knockdowns, labeled with the RLC-D,D–GFP) that form and disassemble rapidly. Images were captured in TIRF mode using an inverted microscope (IX70; Olympus) coupled to a CCD camera (Retiga Exi; Qimaging). Live samples were acquired at 37°C using CCM1 medium. This movie corresponds to Fig. 1 D. and Video 2 from J Cell Biol 2011 193:381-396. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Vicente-Manzanares, Miguel; Newell-Litwa, Karen; Bachir, Alexia I.; Whitmore, Leanna A.; Horwitz, Alan Rick (2021). CIL:25844, Cricetulus griseus, epithelial cell. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0H130W7
Type
image
Identifier
ark:/20775/bb06210064
Language
No linguistic content
Subject
CHO-K1 Myosin IIB depletion Phospho-mimetic Epithelial cell Myosin II complex Cricetulus griseus Cell Image Library Group ID: 7724
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