Under copyright Constraint(s) on Use: This work is protected by the U.S. Copyright Law (Title 17, U.S.C.). Use of this work beyond that allowed by "fair use" or any license applied to this work requires written permission of the copyright holder(s). Responsibility for obtaining permissions and any use and distribution of this work rests exclusively with the user and not the UC San Diego Library. Inquiries can be made to the UC San Diego Library program having custody of the work. Use: This work is available from the UC San Diego Library. This digital copy of the work is intended to support research, teaching, and private study.
Rights Holder and Contact
UC Regents
Description
Spinning disk confocal time-lapse imaging of the first mitosis in a C. elegans embryo that has been depleted of KNL-2 by RNAi. GFP-histone and GFP-γ-tubulin were imaged (strain TH32). Pronuclear migration is slightly disrupted by a chromatin bridge resulting from failed meiotic segregation of the maternal chromatin. As seen in CeCENP-A depletion (CIL 28776), chromosome condensation is disrupted, chromosomes fail to form a proper metaphase plate, and the spindle poles move away from the chromatin masses prematurely. Anaphase segregation fails, and chromatin masses are left in the middle of the cell. Also note the meiotic defect that results in a defective oocyte pronucleus (left side of image at the beginning of the videos; this is the embryo anterior). The video is ∼16 min long, and the frame is ∼30 µm top to bottom. Images were acquired at 10-s intervals. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Maddox, Paul S.; Hyndman, Francie; Monen, Joost; Oegema, Karen; Desai, Arshad (2021). CIL:28777, Caenorhabditis elegans, embryo. In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J0BZ64P3
Type
image
Identifier
ark:/20775/bb0825842x
Language
No linguistic content
Subject
KNL-2 depletion Mitosis Nuclear chromosome Gamma tubulin Embryo Chromatin bridge Spindle pole Caenorhabditis elegans Cell Image Library Group ID: 11164
If you're wondering about permissions and what you can do with this item, a good starting point is the "rights information" on this page. See our terms of use for more tips.
Share your story
Has Calisphere helped you advance your research, complete a project, or find something meaningful? We'd love to hear about it; please send us a message.
