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Description
This is IMAGE #2 (4 hours) of a time lapse series contained with in this image group. It shows neurons from the optic tectum of an albino Xenopus laevis tadpole CNS, stage 47. The pigment cells in the skin have high autofluorescence and are visible in the superficial layers of the z-stack. The images were acquired at 4 hour intervals over 8 hours, and show morphological plasticity of the dendritic arbor with branch additions and retractions. These cells were transfected with in vivo electroporation of plasmid DNA encoding eGFP ~24 hours before the first image was acquired. The intact, living tadpole was anesthetized, positioned under a glass coverslip and this image was taken directly through its transparent skin using a custom built two-photon microscope. Research Data Curation Program, UC San Diego, La Jolla, 92093-0175 (https://lib.ucsd.edu/rdcp) Bestman, Jennifer; Cline, Holly (2022). CIL:37044, Xenopus laevis, CNS neuron (sensu Vertebrata). In Cell Image Library. UC San Diego Library Digital Collections. Dataset. https://doi.org/10.6075/J06Q1X4W
Type
image
Identifier
ark:/20775/bb10653637
Language
No linguistic content
Subject
Neuron development Morphogenesis of a branching structure CNS neuron (sensu Vertebrata) Dendrite Neuronal cell body Cytoplasm Axon Xenopus laevis Cell Image Library Group ID: 9256
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